Macrophage migration inhibitory factor promotes the migration of dendritic cells through CD74 and the activation of the Src/PI3K/myosin II pathway

Constitutively expressed by innate immune cells, the cytokine macrophage migration inhibitory factor (MIF) initiates host responses and drives pathogenic in infectious, inflammatory, autoimmune diseases. Dendritic cells (DCs) express high levels of MIF, but role MIF DC function remains poorly characterized. As is critical for surveillance, we investigated whether promoted DCs. In classical transwell experiments, MIF?/? bone marrow-derived DCs (BMDCs) or MIF+/+ BMDCs treated with ISO-1, an inhibitor showed markedly reduced spontaneous chemotaxis. CD74?/? that are deficient ligand-binding component cognate receptor exhibited a defect similar to BMDCs. Adoptive transfer experiments LPS-matured CD74+/+ injected into hind footpads homologous heterologous mice autocrine paracrine activity acting via CD74 contributed recruitment draining lymph nodes. Mechanistically, activated Src/PI3K signaling pathway myosin II complexes, which were required Altogether, these data show exerts chemokine-like motility trafficking.